梁国嫔²，彭睿妍¹，曹海燕³，阙素云³，张清峰^1*

（1.江西农业大学食品科学与工程学院，江西省天然产物与功能食品重点实验室，南昌 330045；2.江西省药品检查员中心，吉安 331500；3.江西维莱营健高科有限公司，吉安 331500）

摘要：建立了山楂中四种黄酮成分（芦丁、牡荆素、金丝桃苷和槲皮素）的高效液相色谱分析方法。采用Agilent 5 TC-C18色谱柱 (250 mm×4.6 mm，5 μm)，流动相为乙腈(A)和0.2%醋酸水溶液(B)，流速为1 mL/min，进样量为10μL，检测波长为355nm，柱温为40℃。线性梯度洗脱程序：0～15min，20%～30% A；15～30min，30%～60% A。四种成分线性关系良好；日内精密度保留时间RSD均＜0.5%，峰面积RSD均＜2.5%；日间精密度保留时间RSD均＜0.5%，峰面积RSD均＜5.0%；平均加样回收率为103.4%～106.7%，RSD均＜2.5%。60%乙醇为最佳提取溶剂。6批山楂样品中芦丁和金丝桃苷的含量范围分别为51.8～264.5 μg/g和148.0～441.6 μg/g，槲皮素只在两批样品中检出，分别为11.0μg/g和12.7μg/g，牡荆素在所有山楂样品中均未检出。

关键词：山楂；高效液相色谱法；黄酮

中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2024）04-0104-0005

Analysis of Four Flavonoids in Hawthorn Fruit by HPLC

LIANG Guopin²，PENG Ruiyan¹，CAO Haiyan³，QUE Suyun³，ZHANG Qingfeng^1*

（1.Jiangxi Key Laboratory of Natural Product and Functional Food，College of Food Science and Engineering，Jiangxi Agriculture University，Nanchang 330045，China；2.Jiangxi Drug Inspection Center，Ji'an 331500，China；3.Jiangxi Weilaiyingjian High-tech Co.，Ltd.，Ji'an 331500，China）

Abstract：A HPLC method was developed to determine four flavonoids in hawthorn fruit， including rutin， vitexin， hypericin and quercetin. An Agilent 5 TC-C18 column (250 mm×4.6 mm， 5 μm) was used with the mobile phases consisting of acetonitrile (A) and 0.2% acetic acid (B) at the flow rate of 1 mL/min. The injection volume was 10 μL，while the detection wavelength was set at 355 nm and the column temperature was 40 ℃. The linear gradient elution procedure was 20%～30% A in 0～15 min， and 30%～60% A in 15～30 min. Methodological results showed that the four components have a good linear relationship. The intraday precision retention time RSD was＜0.5%， and the peak area RSD was＜2.5%. The RSD of interday precision retention time was＜0.5% and the RSD of peak area was ＜5.0% . The average recoveries ranged from 103.4% to 106.7%， and the RSD was＜2.5%. The optimal extraction solvent was 60% ethanol. The contents of rutin and hypericin in 6 batches of hawthorn samples were in the range of 51.8～264.5 μg/g and 148.0～441.6 μg/g， respectively. Quercetin was detected in 2 batches of hawthorn samples， with contents of 11.0 and 12.7 μg/g， and vitexin was undetectable in all samples.

Keywords：hawthorn fruit；high performance liquid chromatography；flavonoid

doi：10.3969/j.issn.1674-506X.2024.04-017

收稿日期：2023-08-07

基金项目：国家自然科学基金项目（32060541）

作者简介：梁国嫔（1977-），女，副主任药师。研究方向：药物分析。

*通信作者：张清峰（1983-），男，教授。研究方向：功能食品成分。

引用格式：梁国嫔，彭睿妍，曹海燕，等. 高效液相色谱法分析山楂中的四种黄酮成分［J］. 食品与发酵科技，2024，60（4）：104-108.